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KMID : 1149220120200020013
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Korean Journal of Oriental Medical Prescription
2012 Volume.20 No. 2 p.13 ~ p.28
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Experimental Studies on the inflammation-related diseases pharmacological effect of water and 70% ethanol extracts from Socheongnyong-tang
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Jeon Woo-Young
Lee Mee-Young
Lim Hye-Sun
Shin In-Sik
Kim Ye-Ji
Jin Seong-Eun
Yoo Sae-Room
Seo Chang-Seob
Kim Jung-Hoon
Ha Hye-Kyung
Kim Ohn-Soon
Shin Na-Ra
Kim Seong-Sil
Shin Hyeun-Kyoo
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Abstract
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Objectives : The present study aimed to investigate the pharmacological activity of water and ethanol (EtOH) extracts from Socheongnyong-tang (SCNT) on inflammation and its related disease.
Methods : The cells were treated with nontoxic concentrations of water and EtOH extract from SCNT in BEAS-2B, HaCaT, RAW 264.7 and 3T3-L1 cells. These cells were stimulated by tumor necrosis facter (TNF)-α, TNF-α/interferon (IFN)-γ, and lipopolysaccharide (LPS), respectively. 3T3-L1 cells were differentiated by insulin. After incubation, supernatant were collected and biological indicator measured by enzyme-linked immunosorbent assay.
Results : Our results indicate that the water and EtOH extract of SCNT significantly inhibited the production of regulated on activation normal T-cell expression and secreted (RANTES) by treatment of TNF-α in BEAS-2B cell, and significantly reduced the production of RANTES and macrophage-derived chemokine increased by treatment of TNF-α/IFN-γ in HaCaT cell. Moreover, those extracts significantly decreased the activity of nitric oxide and prostaglandin E2 in LPS-induced RAW 264.7, and significantly inhibited the increased activity of glycerol-3-phosphate dehydrogenase and expression of leptin induced by differentiation in 3T3-L1 cell.
Conclusions : These results indicate that both water and EtOH extract of SCNT has powerful effects on inflammation and its related disease. Therefore, SCNT can be developed as a potential pharmacological agent related various diseases. Although the significant effects were observed in both SCNT water and EtOH extract, the EtOH extract was more effective on most experiments than its water extract. Taken together, these findings indicate that the SCNT EtOH extract may have more potential pharmacological agent.
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KEYWORD
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Socheongnyong-tang, Asthma, Allergy, Inflammation, Obesity
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